Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3-FLAG-hRIP4-K158R (LMBP 6716)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human receptor-interacting serine-threonine kinase 4 cDNA (RIPK4, RIP4, ANKRD3, ANKK2, DIK, PKK); mutated sequence FLAG epitope tag; N-terminal |
Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pcDNA3 |
Further information: | The plasmid was constructed by cloning the FLAG-tagged human RIP4 mutated sequence into the pcDNA3 vector. The human RIP4 coding sequence contains a K158R mutation. The nucleotide sequence of the wild type human RIP4 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number BC110617.1. Other name of the plasmid is pcDNA-Flag-hRIP4 K158R. |
EMBL Accession number: | BC110617.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 02/09/2010 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr W. Declercq(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Related plasmid reference: | Bertrand et al., PLoS One. 6 (2011), e22356 [PMID: 21931591] [DOI: 10.1371/journal.pone.0022356] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3-FLAG-hRIP4-K158R (LMBP 6716) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Declercq . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.