Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3-HA-hDRD4-2(∆290-295) (LMBP 10559)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10559.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human dopamine receptor D4 cDNA (DRD4, GeneID 1815); synthetic fragment (DRD4-2) Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3 |
| Further information: | The plasmid was constructed by cloning the HA-tagged synthetic human DRD4-2 coding sequence, lacking codons 290 up to and including 295, into the pcDNA3 vector. The synthetic human DRD4-2 coding sequence was designed by Snyder & Sakmar (unpublished). It was derived from D4 receptor variant sequences presented in GenBank Accession Number L12398 and contains a direct repeat (D4-2) of a 48 basepair sequence in the putative third cytoplasmic loop. The sequence has been altered by inserting new restriction sites while maintaining the original amino acid sequence. Codons 290 up to and including 295 have been removed from the coding sequence. The nucleotide sequence of the synthetic human DRD4-2 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF119328.1. Other names of the plasmid are pcDNA3HAD4.2(∆290-295) and pcDNA3-HA-hDRD4-2(d290-295). |
| EMBL Accession number: | AF119328.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 28/11/2018 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr K. Van Craenenbroeck(1). (1) Laboratory for GPCR Expression and Signal Transduction, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3-HA-hDRD4-2(∆290-295) (LMBP 10559) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. Van Craenenbroeck. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.