Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3-myc-mDNp63aQ536L (LMBP 8094)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8094.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse transformation related protein 63 (Trp63); transactivation inhibitor isoform alpha (DNp63a, ∆Np63a), mutated sequence Myc epitope; N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3-myc-mDNp63a |
| Further information: | The plasmid was constructed by introducing a Q536L mutation into the mouse DNp63a coding sequence of pcDNA3-myc-mDNp63a. Amino acid numbering is based on known mutations of the human p63 gene. Therefore the numbering of the mutation does not correspond with the amino acid position in the mouse homologues. The nucleotide sequence of the wild type mouse DNp63a coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF075439.1. Other name of the plasmid is pcDNA3-myc-mDNp63aG536L. |
| EMBL Accession number: | AF075439.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 25/01/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr Hans Van Bokhoven(1) and Prof. Dr Frank McKeon(2). (1) Molecular Neurogenetics, Department of Human Genetics, Radboud university medical center, Nijmegen, Netherlands (2) Somatic Stem Cell Center, Department of Biology and Biochemistry, University of Houston, Houston, USA |
| Plasmid reference: | - |
| Related plasmid reference: | PhD thesis Michael Devos (2012) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3-myc-mDNp63aQ536L (LMBP 8094) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Hans Van Bokhoven and Prof. Dr Frank McKeon. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.