Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3.1-EGFP-mMyD88 (LMBP 5644)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5644.gb
(View with Genome Compiler) p5644.txt p5644.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse myeloid differentiation primary response gene 88 cDNA (Myd88, GeneID 17874) Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP) |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAGGS-E-mMyD88; pcDNA3-GFP-Inca |
| Further information: | The mMyd88 coding sequence from pCAGGS-E-mMyD88 was cloned via KpnI/ApaI in pcDNA3-GFP-Inca, in frame with the N-terminal EGFP coding sequence. The plasmid is designed for high-level expression of EGFP-mMyd88 under control of the strong hCMV-IE promoter and enhancer in mammalian cells. There is uncertainty about the presence of a second enhancer in the SV40 early promoter. The absence of a second enhancer in this promoter may cause bacterial leakage expression of the Tn5 neomycin resistance gene. This would cause transformed E. coli cells to be resistant to kanamycin, although growth should be reduced compared to growth on medium containing ampicillin. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726937.1. The nucleotide sequence of the mMyd88 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U84409.1. The nucleotide sequence of the EGFP DNA corresponds with the EMBL Nucleotide Sequence Database accession number U55761.1. Other name of the plasmid is pcDNA3-GFP-mMyD88. |
| EMBL Accession number: | U84409.1, view at EMBL, GenBank, DDBJ U55761.1, view at EMBL, GenBank, DDBJ LT726937.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/03/2009 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: ApaI/KpnI, BssHII/PstI, Bst98I/NdeI, EcoRV, HindIII/XmnI, NaeI/NarI, NcoI, PvuII/XmaI and SacI/SmaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr S. Janssens(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Janssens et al., Trends Biochem. Sci. 27 (2002), 474-482 [PMID: 12217523] Janssens et al., FEBS Lett. 548 (2003), 103-107 [PMID: 12885415] Janssens et al., Curr. Biol. 12 (2002), 467-471 [PMID: 11909531] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3.1-EGFP-mMyD88 (LMBP 5644) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Janssens and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.