Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3.1-Flag-His-ATM (LMBP 6463)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6463.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human ATM serine/threonine kinase cDNA (ATM, TEL1, TELO1, GeneID 472) |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3.1 |
| EMBL Accession number: | - |
| Latest sequence update: | 01/10/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr M. Kastan(1). (1) Duke Cancer Institute, Durham, USA |
| Plasmid reference: | Canman et al., Science 281 (1998), 1677-1679 [PMID: 9733515] [DOI: 10.1126/science.281.5383.1677] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | Because ATM tends to recombine, the plasmid-carrying E. coli strain should be cultivated at 28°C. You should also pick the small colonies on the amp plates after 48h (and not the earlier developing larger colonies which represent recombined ATM). Therefore the plasmid integrity should be confirmed by NcoI digestion after each subcultivation. |
| Other culture collection numbers: | Addgene 31985 |
Refer in your Materials and Methods: |
pcDNA3.1-Flag-His-ATM (LMBP 6463) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr M. Kastan and was published in Canman et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.