Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3.1-Flag-His6-hNEMO-2Ub (LMBP 9751)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma cDNA (IKBKG, IP1, IP2, FIP-3, FIP3, Fip3p, IKK-gamma, NEMO, ZC2HC9) Human ubiquitin B cDNA (UBB, Ub, HEL-S-50, GeneID 7314) Human ubiquitin B cDNA (UBB, Ub, HEL-S-50, GeneID 7314); mutated sequence FLAG epitope tag; N-terminal Histidine tag (His-tag); N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3.1-Flag |
| Further information: | The plasmid was constructed by cloning the His-tagged human IKBKG coding sequence, fused to two human Ub coding sequences, into the pcDNA3.1-Flag vector. Two human Ub coding sequences are fused to the C-terminus of the human IKBKG coding sequence. In the second Ub coding sequence, the two glycines are exchanged for valines to prevent conjugation. The Ub-GG-Ub complex might be recognised by DUB, causing it to be cleaved in cells, however the efficiency of this cleavage is unknown. Other name of the plasmid is pcDNA3.1 FLAG-His6-NEMO-ub2. |
| EMBL Accession number: | - |
| Latest sequence update: | 11/01/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr I. Dikic(1). (1) Institute of Biochemistry II, Goethe University School of Medicine, Frankfurt, Germany |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3.1-Flag-His6-hNEMO-2Ub (LMBP 9751) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr I. Dikic. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.