Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3.1-Flag-TANKdeltaC50 (LMBP 5548)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5548.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human TRAF family member associated NFKB activator (TANK, TRAF2, I-TRAF, GeneID 10010); fragment FLAG epitope tag; N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3.1-Flag-TANK |
| Further information: | The plasmid was constructed by PCR amplifying the truncated hTANK cDNA from pcDNA3.1-Flag-TANK and replacing the full length hTANK cDNA in the parental vector. The truncated hTANK cDNA is missing the 50 C-terminal codons. The plasmid is meant as a eukaryotic expression vector. There is uncertainty about the presence of a second enhancer in the SV40 early promoter. The absence of a second enhancer in this promoter may cause bacterial leakage expression of the Tn5 neomycin resistance gene. This would cause transformed E. coli cells to be resistant to kanamycin, although growth should be reduced compared to growth on medium containing ampicillin. The nucleotide sequence of the full length hTANK cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U59863.1. |
| EMBL Accession number: | U59863.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 13/05/2009 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: ApaI/HaeII, HindIII/XbaI and PvuI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr. A. Chariot(1). (1) Laboratory of Medical Chemistry, Liege University, Liege, Belgium |
| Plasmid reference: | Bonif et al., Biochem. J. 394 (2006), 593-603 [PMID: 16336209] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3.1-Flag-TANKdeltaC50 (LMBP 5548) is available at BCCM/GeneCorner. This plasmid was deposited by Dr. A. Chariot and was published in Bonif et al., 2006. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.