Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3.1-HA-XTRAF4deltaRING (LMBP 6132)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6132.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Xenopus laevis TNF receptor associated factor 4 cDNA (TRAF4); fragment Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Synthetic prokaryotic EM7 promoter Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Zeocin (zeo) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3.1/Zeo(+) |
| Further information: | The plasmid was constructed by cloning the HA-tagged Xenopus TRAF4 coding sequence, lacking the N-terminal RING domain (57 AA), including the 3' UTR region, into the EcoRV/XbaI opened pcDNA3.1/Zeo(+) vector. The nucleotide sequence of the Xenopus TRAF4 cDNA corresponds with EMBL Nucleotide Sequence Database accession number BC080018.1. |
| EMBL Accession number: | BC080018.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/01/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr Gerald H. Thomsen(1). (1) Department of Biochemistry and Cell Biology, Center for Developmental Genetics, Stony Brook University, Stony Brook, USA |
| Plasmid reference: | Kalkan et al., Mol. Biol. Cell 20 (2009), 3436-3450 [PMID: 19458200] [DOI: 10.1091/mbc.E08-03-0325] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3.1-HA-XTRAF4deltaRING (LMBP 6132) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Gerald H. Thomsen and was published in Kalkan et al., 2009. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.