Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3zeo-F-HOIL-Strep (LMBP 7317)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7317.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human RANBP2-type and C3HC4-type zinc finger containing 1 cDNA (RBCK1, HOIL1, RBCK2, RNF54, UBCE7IP3, XAP4, ZRANB4, GeneID 10616) FLAG epitope tag; N-terminal Strep-tag II; C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter Synthetic prokaryotic EM7 promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCDNA3-zeo; pUC52-HOIL |
| Further information: | The plasmid was constructed by isolating the FLAG- and Strep-tagged synthetic human RBCK1 coding sequence from pUC52-HOIL and cloning it into the XhoI/HindIII opened pCDNA3-zeo vector. The nucleotide sequence of the human RBCK1 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number BC015219.2. |
| EMBL Accession number: | BC015219.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/07/2017 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindIII/KpnI, NcoI and PvuII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr J. Staal(1)(2) and Prof. Dr R. Beyaert(1)(2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Elton et al., FEBS J. 283 (2016), 403-412 [PMID: 26573773] [DOI: 10.1111/febs.13597] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3zeo-F-HOIL-Strep (LMBP 7317) is available at BCCM/GeneCorner. The plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.