Last data update: 24 January 2024 16:39 CET
Plasmid name: pcI857Cam (LMBP 1769)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1769.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage λ cI temperature-sensitive repressor gene (cIts, cI857) |
| Promoter: | Phage λ major rightward promoter (λ PR) Phage λ promoter for repressor maintenance (λ PRM) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Chloramphenicol (cam; contains an amber codon) |
| Replicon: | Escherichia coli plasmid p15A origin |
| Copy number: | Low copy number |
| Host range: | Escherichia coli; use strains with an amber sup(+) character (preferable supF). |
| Parental clone: | pcI857; pMa58 |
| Further information: | This plasmid was derived from pcI857 by inserting the SmaI-XmnI (Asp700) fragment of pMa58 into the unique SmaI site of pcI857. The orientation of the fragment between nucleotide 4 and 57 is unknown. The kanamycin resistance gene of pcI857 is inactivated by insertion of the chloramphenicol resistance gene of pMa58, which contains an amber termination codon; so, this plasmid has to be selected in sup(+) strains. |
| EMBL Accession number: | - |
| Latest sequence update: | 25/03/2011 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by E. Sablon(1) and Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 QD5003 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcI857Cam (LMBP 1769) is available at BCCM/GeneCorner. This plasmid was deposited by E. Sablon and Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.