Last data update: 24 January 2024 16:39 CET
Plasmid name: pdCS3-maTctn(120-2979) (LMBP 5329)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human αT-catenin cDNA (aTctn, αTctn, CTNNA3); fragment Myc epitope; N-terminal |
| Promoter: | Simian cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells Avian cells |
| Parental clone: | pdCS3; pENTR2B-maTctn(120-2979) |
| Further information: | This Gateway expression vector was obtained by Gateway cloning the mouse α-T-catenin from the plasmid pENTR2B-maTctn(120-2979) into the pdCS3 vector using the LR-clonase enzyme. The pdCS3 vector was constructed by inserting the Gateway conversion cassette A (Invitrogen) into the StuI site of plasmid pCS3. The nucleotide sequence of the mCTNNA3 cDNA was obtained from Genbank (Accession number; AF344871.1). Other name of the plasmid is pdCS3 maTctn FL(120-2979). |
| EMBL Accession number: | AF344871.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/03/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by S. Goossens(1) (2) and Prof. Dr F. Van Roy(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Busby et al., Neuromolecular Med. 5 (2004), 133-46 [PMID: 15075440] |
| Related plasmid reference: | Goossens et al., J. Cell Sci. 120 (2007), 2126-2136 [PMID: 17535849] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pdCS3-maTctn(120-2979) (LMBP 5329) is available at BCCM/GeneCorner. This plasmid was deposited by S. Goossens and Prof. Dr F. Van Roy and was published in Busby et al., 2004. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.