Last data update: 24 January 2024 16:39 CET
Plasmid name: pdGADT7-hCARD9 (LMBP 9878)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9878.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human caspase recruitment domain family member 9 cDNA (CARD9, GeneID 64170) Saccharomyces cerevisiae GAL4 activation domain (GALad) Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) |
| Selection marker: | Ampicillin (amp) LEU2; auxotrophic |
| Replicon: | Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-) |
| Parental clone: | pDEST-GADT7; pENTR3C-hCARD9 |
| Further information: | This Yeast-2-hybrid Gateway expression plasmid was constructed by cloning the human CARD9 coding sequence from pENTR3C-hCARD9 into the pDEST-GADT7 vector via Gateway LR cloning. The nucleotide sequence of the parental pDEST-GADT7 vector corresponds with the EMBL Nucleotide Sequence Database accession number LT727257.1. |
| EMBL Accession number: | LT727257.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 01/10/2018 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Staal et al., bioRxiv (2016), 1-42 [DOI: 10.1101/046789] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pdGADT7-hCARD9 (LMBP 9878) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert and was published in Staal et al., 2016. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.