Last data update: 24 January 2024 16:39 CET
Plasmid name: pdGBKT7-TaCdh1-cyto (LMBP 12674)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Trichoplax adhaerens cadherin 1 cDNA (Cdh1); fragment Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) Myc epitope; N-terminal |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli β-lactamase promoter (amp) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Neomycin (neo; kanamycin (kan)) TRP1; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) Phage f1 origin |
| Host range: | Escherichia coli Saccharomyces cerevisiae; trp1(-) |
| Parental clone: | pGBKT7 |
| Further information: | The plasmid was constructed by cloning a C-terminal fragment of the T. adhaerens Cdh1 coding sequence, starting at nucleotide 12316, into the pGBKT7 vector. Other name of the plasmid is pdGBKT7_Ta_Ecad cyto cl11. |
| EMBL Accession number: | - |
| Latest sequence update: | 25/10/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by K. Staes(1)(2) and Prof. Dr F. Van Roy(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + kanamycin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pdGBKT7-TaCdh1-cyto (LMBP 12674) is available at BCCM/GeneCorner. The plasmid was deposited by K. Staes and Prof. Dr F. Van Roy. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.