Last data update: 24 January 2024 16:39 CET
Plasmid name: pdGBKT7-hVCL-VH2+IN2+VH3+Tail (LMBP 12565)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human vinculin cDNA (VCL, GeneID 7414); fragment Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) Myc epitope; N-terminal |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli β-lactamase promoter (amp) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Neomycin (neo; kanamycin (kan)) TRP1; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) Phage f1 origin |
| Host range: | Escherichia coli Saccharomyces cerevisiae; trp1(-) |
| Parental clone: | pDEST-GBKT7; pENTR207-hVCL-VH2+IN2+VH3+Tail |
| Further information: | This Yeast-2-hybrid Gateway expression plasmid was constructed by cloning the human VCL coding sequence from pENTR207-hVCL-VH2+IN2+VH3+Tail into the pDEST-GBKT7 vector via Gateway LR cloning. The plasmid encodes the head domain VH2, the linker peptide IN2, the head domain VH3 and the tail domain of human VCL. Other name of the plasmid is pdGBKT7 hVinculin VH2+IN2+VH3+Tail. |
| EMBL Accession number: | - |
| Latest sequence update: | 11/10/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by K. Staes(1)(2) and Prof. Dr F. Van Roy(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pdGBKT7-hVCL-VH2+IN2+VH3+Tail (LMBP 12565) is available at BCCM/GeneCorner. The plasmid was deposited by K. Staes and Prof. Dr F. Van Roy. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.