Last data update: 24 January 2024 16:39 CET
Plasmid name: pdGBKT7-mPcdh11-noRVTF (LMBP 7811)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse protocadherin 11 X-linked cDNA (Pcdh11x, GeneID 245578); fragment Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) Myc epitope; N-terminal |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Phage T7 gene 10 promoter (T7g10) Escherichia coli β-lactamase promoter (amp) Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) TRP1; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) Phage f1 origin |
| Host range: | - |
| Parental clone: | pdGBKT7 |
| Further information: | This Gateway expression vector was constructed by cloning a fragment of the mouse Pcdh11x coding sequence into the pdGBKT7 vector via Gateway LR recombination. The plasmid encodes the cytoplasmic domain of the short isoform of mouse Pcdh11x. Other name of the plasmid is pdGBKT7-mPcdh11 noRVTF cl2. |
| EMBL Accession number: | - |
| Latest sequence update: | 15/03/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr F. Van Roy(1) (2). It was constructed by K. Vandepoele(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pdGBKT7-mPcdh11-noRVTF (LMBP 7811) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr F. Van Roy . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.