Last data update: 24 January 2024 16:39 CET
Plasmid name: pdWPIteto-hRIP4-K51R-stop-V5-His (LMBP 7909)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7909.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human receptor-interacting serine-threonine kinase 4 cDNA (RIPK4, RIP4, ANKRD3, ANKK2, DIK, PKK); mutated sequence V5 epitope; C-terminal Histidine tag (His-tag); C-terminal Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP) Escherichia coli gpt gene encoding xanthine-guanine phosphoribosyltransferase (XGPRT) |
| Promoter: | Human elongation factor 1α promoter (EF1α) Simian virus 40 early promoter (SV40 early) Phage SP6 promoter Rous sarcoma virus/human immunodeficiency virus hybrid long terminal repeat (RSV/HIV-1 5' LTR) |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Human immunodeficiency virus long terminal repeat (HIV-1 3' LTR); self-inactivating chimeric LTR with loxP insert |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pdWPIteto-V5His |
| Further information: | This lentiviral Gateway expression vector was constructed by cloning the mutated human RIP4 coding sequence into the pdWPIteto-V5His vector via Gateway recombination. The human RIP4 coding sequence contains a K51R mutation and lacks a termination codon, allowing for C-terminal V5- and His-tagging. This lentiviral Gateway expression vector allows for Tet inducible expression of the V5-His-tagged human RIP4 mutant. The plasmid contains: - the HIV-1 psi packaging signal - the HIV-1 Rev response element - the Tet-responsive element (TRE), that consists of seven copies of the 42 bp tetracycline operator O2 of E. coli Tn10 (tetO), each containing a 19 bp inverted repeat sequence - the post-transcriptional regulatory element (PRE) of woodchuck hepatitis virus (WHV), that enhances both titer and transgene expression when introduced into the 3' untranslated region of retroviral and lentiviral vectors. The nucleotide sequence of the wild type human RIP4 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number BC110617.1. Other name of the plasmid is pdWPIteto hRIP4K51R-S V5His. |
| EMBL Accession number: | BC110617.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/01/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Declercq(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 in E. coli; L2 in mammalian cells |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pdWPIteto-hRIP4-K51R-stop-V5-His (LMBP 7909) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Declercq . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.