Last data update: 24 January 2024 16:39 CET
Plasmid name: pdcDNA3-FLAG-hRIP4-stop-Myc (LMBP 7897)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7897.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human receptor-interacting serine-threonine kinase 4 cDNA (RIPK4, RIP4, ANKRD3, ANKK2, DIK, PKK) FLAG epitope tag; N-terminal Myc epitope; C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pdcDNA-FLAGMyc |
| Further information: | This plasmid was created by inserting the human RIP4 coding sequence without termination codon into the pdcDNA-FLAGMyc vector by Gateway recombination. The myc epitope in this Gateway expression vector is not functional. The nucleotide sequence of the human RIP4 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number BC110617.1. Other name of the plasmid is pcDNAflag_hRIP4_myc kl3. |
| EMBL Accession number: | BC110617.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/01/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Declercq(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pdcDNA3-FLAG-hRIP4-stop-Myc (LMBP 7897) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Declercq . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.