Last data update: 24 January 2024 16:39 CET
Plasmid name: pdcDNA3-strep-NLS-hRIP3-K50A (LMBP 10539)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10539.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human receptor interacting serine/threonine kinase 3 cDNA (RIPK3, RIP3, GeneID 11035); mutated sequence Strep-tag II; N-terminal SV40 large T-antigen nuclear localization signal (NLS); N-terminal |
| Promoter: | Escherichia coli lac operon promoter Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Phage f1 origin Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pENTR3C-NLS-hRIP3-K50A; pdcDNA3-strep |
| Further information: | This Gateway expression vector was constructed by cloning the human RIPK3 mutant, preceded by the SV40 NLS signal and including its termination codon, into the pdcDNA3-strep vector via Gateway LR recombination with pENTR3C-NLS-hRIP3-K50A. The human RIPK3 coding sequence contains a K50A mutation. The nucleotide sequence of the wild type human RIPK3 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF156884.1. Other name of the plasmid is pdcDNA3 - strep - NLS - hRIP3K50A + stop. |
| EMBL Accession number: | AF156884.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/11/2018 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr P. Vandenabeele(1)(2). It was constructed by I. Bruggeman(1)(2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pdcDNA3-strep-NLS-hRIP3-K50A (LMBP 10539) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr P. Vandenabeele. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.