Last data update: 24 January 2024 16:39 CET
Plasmid name: pdl3027 (LMBP 2310)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pdl3027.gb
(View with Genome Compiler) pdl3027.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Polyoma strain A2; with deletion |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pAT153 |
| Further information: | The plasmid was constructed as follows: polyoma (strain A2 with a deletion of 110 nucleotides at the origin sequence) was BamHI opened, and inserted into the unique BamHI site of pAT153. The orientation of the polyoma fragment was confirmed by restriction enzyme analysis. This plasmid is used to transfect mammalian cells, in order to make them permissive for vectors containing the polyoma 'ori', such as pCDM8. |
| EMBL Accession number: | - |
| Latest sequence update: | 16/06/1992 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: ApaI, Bst98I, HindII and HindIII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr C. Basilico(1). (1) New York University School of Medicine, New York |
| Plasmid reference: | Tyndall et al., Nucleic Acids Res. 9 (1981), 6231-6250 [PMID: 6275353] |
| Related plasmid reference: | Dailey et al., J. Virol. 54 (1985), 739-749 [PMID: 2987528] Cowie et al., J. Virol. 52 (1984), 750-760 [PMID: 6092707] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pdl3027 (LMBP 2310) is available at BCCM/GeneCorner. This plasmid was deposited by Dr C. Basilico and was published in Tyndall et al., 1981. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.