Last data update: 24 January 2024 16:39 CET
Plasmid name: phasmid FlpE (LMBP 11285)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
NGS: d02-gc-dec2019.fasta |
| Cloned DNA: |
Saccharomyces cerevisiae 2μ plasmid site-specific recombinase Flp cDNA (FLP1); enhanced Flp recombinase (FlpE) Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal Phage TM4 genome; cDNA |
| Promoter: | Mycobacterium tuberculosis intermediate strong promoter (Pimyc) Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli (as a plasmid) Mycobacterium (as a phage) |
| Parental clone: | phAE159; suicide vector FlpE |
| Further information: | This phasmid was constructed by isolating the transposon cassette from suicide vector FlpE as a PacI fragment and ligating it into the PacI opened phAE159 phasmid. Use a lambda packaging protocol to transform E. coli. phAE159 is a temperature-sensitive TM4-based Mycobacterial shuttle plasmid. The nucleotide sequence of the phAE159 phasmid corresponds with the EMBL Nucleotide Sequence Database accession number AF068845.1, except for a number of nucleotide substitutions shown by NGS. |
| EMBL Accession number: | AF068845.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/11/2020 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | - |
| Plasmid reference: | Borgers et al., mSystems 5 (2020), e00180-20 [PMID: 32788404] [DOI: 10.1128/mSystems.00180-20] |
| Restricted distribution: | - |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + hygromycin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
phasmid FlpE (LMBP 11285) is available at BCCM/GeneCorner. - and was published in Borgers et al., 2020. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.