Last data update: 24 January 2024 16:39 CET
Plasmid name: piCA3 (LMBP 6250)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage P22 repressor gene (c2) Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) Phage P22 antirepressor gene (ant) Escherichia coli plasmid pSC101 replication protein A (repA) |
| Promoter: | Escherichia coli lac repressor promoter (lacI) Phage T5 N25 promoter with two lac operator sequences (N25/O2) Phage 22 major rightward promoter (P22 PR) Phage 22 promoter for repressor maintenance (P22 PM) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Kanamycin (kan) |
| Replicon: | Plasmid pSC101 origin derived from R6-5 |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | piCA2 |
| Further information: | The plasmid was constructed by replacing the phage λ cI857 repressor gene in the piCA2 vector by the phage P22 c2 repressor gene and replacing the phage λ PR/PRM promoters with the phage P22 PR/PM promoters. - |
| EMBL Accession number: | - |
| Latest sequence update: | 26/03/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by J. Haustraete(1) (2). (1) Protein Core, VIB-UGent Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
piCA3 (LMBP 6250) is available at BCCM/GeneCorner. This plasmid was deposited by J. Haustraete . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.