Last data update: 24 January 2024 16:39 CET
Plasmid name: piCAS02 (LMBP 6263)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) Phage P22 antirepressor gene (ant) Escherichia coli plasmid pSC101 replication protein A (repA) Phage λ cI temperature-sensitive repressor gene (cIts, cI857) Escherichia coli DNA-binding transcriptional regulator AraC (araC) Escherichia coli Sec translocon subunit SecY cDNA (secY, prlA, GeneID 947799); PrlA4 mutant |
| Promoter: | Escherichia coli lac repressor promoter (lacI) Phage λ major rightward promoter (λ PR) Phage λ promoter for repressor maintenance (λ PRM) Escherichia coli L-arabinose operon promoter (araB, araBAD, BAD) Escherichia coli hybrid tryptophan/lacUV5 promoter (tac) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Kanamycin (kan) |
| Replicon: | Plasmid pSC101 origin derived from R6-5 |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | piCA5 |
| Further information: | The plasmid was constructed by cloning the mutated E. coli secY coding sequence into the piCA5 vector. PrlA4 is one of the strongest prl suppressors and contains two mutations in the secY gene that result in the amino acid substitutions F286Y and I408N. |
| EMBL Accession number: | - |
| Latest sequence update: | 26/03/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by J. Haustraete(1) (2). (1) Protein Core, VIB-UGent Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
piCAS02 (LMBP 6263) is available at BCCM/GeneCorner. This plasmid was deposited by J. Haustraete . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.