Last data update: 24 January 2024 16:39 CET
Plasmid name: piCAS10 (LMBP 6271)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) Phage P22 antirepressor gene (ant) Escherichia coli plasmid pSC101 replication protein A (repA) Phage λ cI temperature-sensitive repressor gene (cIts, cI857) Escherichia coli DNA-binding transcriptional regulator AraC (araC) Escherichia coli murein lipoprotein cDNA (lpp, mlpA, GeneID 946175); secretion signal Escherichia coli bacteriocin release protein cDNA (brp); mutated fragment |
| Promoter: | Escherichia coli lac repressor promoter (lacI) Phage λ major rightward promoter (λ PR) Phage λ promoter for repressor maintenance (λ PRM) Escherichia coli L-arabinose operon promoter (araB, araBAD, BAD) Escherichia coli hybrid tryptophan/lacUV5 promoter (tac) Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Kanamycin (kan) |
| Replicon: | Plasmid pSC101 origin derived from R6-5 |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | piCA5 |
| Further information: | The plasmid was constructed by cloning the E. coli brp C09 mutant coding sequence, preceded by the E. coli lpp signal sequence instead of the native brp signal sequence, into the piCA5 vector. - |
| EMBL Accession number: | - |
| Latest sequence update: | 26/03/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by J. Haustraete(1) (2). (1) Protein Core, VIB-UGent Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
piCAS10 (LMBP 6271) is available at BCCM/GeneCorner. This plasmid was deposited by J. Haustraete . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.