Last data update: 24 January 2024 16:39 CET
Plasmid name: ts vector ISceIM (LMBP 11296)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli galactokinase cDNA (galK, galA, GeneID 945358) Synthetic I-SceI meganuclease cDNA (I-SceI Meganuclease) |
| Promoter: | Mycobacterial optimized promoter (MOP) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin Mycobacterium fortuitum plasmid pAL5000 temperature-sensitive origin (tsOriM) |
| Host range: | Escherichia coli Mycobacterium |
| Parental clone: | pWM18-; pMV261Hyg_GalK_MOP-ISceIM |
| Further information: | The plasmid was constructed by PCR amplifying the temperature sensitive OriM from pWM18- and cloning it into the pMV261Hyg_GalK_MOP-ISceIM vector via Gibson Assembly. This plasmid contains a thermosensitive Mycobacterium origin of replication. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/11/2020 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII/XhoI, BstXI, PvuII, SacII and SalI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1)(2). It was constructed by Dr K. Borgers(1)(2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Borgers et al., mSystems 5 (2020), e00180-20 [PMID: 32788404] [DOI: 10.1128/mSystems.00180-20] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + hygromycin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
ts vector ISceIM (LMBP 11296) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert and was published in Borgers et al., 2020. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.