These Gateway vectors are part of a two-component system for in vivo visualisation of RNA in plant cells.
LMBP 9017 is meant to introduce target RNA, fused to 16 N-terminal boxB loops, which are recognised by the λN22 protein present in one of the corresponding fluorescence markers vectors (LMBP 9019 up to and including 9025).
The λN22/box-B system is well suited for low-background and high-throughput studies.
LMBP 9026 is meant to introduce target RNA, fused to 6 N-terminal MS2 loops, which are recognised by the MS2-CP protein present in one of the corresponding fluorescence markers vectors (LMBP 9028 up to and including 9035).
| You can order plasmids individually, or order the entire group at -10% discount. | Add all plant RNA visualisation plasmids to your shopping cart | ||
| The ccdB-resistant E. coli K12xB DB3.1 strain is also available at BCCM/GeneCorner. | Add E. coli DB3.1 to shopping cart |
| Plasmid name | Depositor's sequence |
| p35S:16xBoxB-Gateway-BASTA | LMBP 9017 |
| p35S:6xMS2-Gateway-BASTA | LMBP 9026 |
| pUBQ10:lambdaN22-CFP-NLS-NPTII | LMBP 9019 |
| pUBQ10:lambdaN22-GFP-NLS-BASTA-RI | LMBP 9020 |
| pUBQ10:lambdaN22-GFP-NLS-NPTII | LMBP 9021 |
| pUBQ10:lambdaN22-mCherry-BASTA | LMBP 9022 |
| pUBQ10:lambdaN22-mCherry-NLS-NPTII | LMBP 9023 |
| pUBQ10:lambdaN22-mVenus-NLS-BASTA | LMBP 9024 |
| pUBQ10:lambdaN22-mVenus-NLS-NPTII | LMBP 9025 |
| pUBQ10:MSCP-CFP-NLS-BASTA | LMBP 9028 |
| pUBQ10:MSCP-CFP-NLS-NPTII | LMBP 9029 |
| pUBQ10:MSCP-GFP-NLS-BASTA | LMBP 9030 |
| pUBQ10:MSCP-GFP-NLS-NPTII | LMBP 9031 |
| pUBQ10:MSCP-mCherry-NLS-BASTA | LMBP 9032 |
| pUBQ10:MSCP-mCherry-NLS-NPTII | LMBP 9033 |
| pUBQ10:MSCP-mVenus-NLS-BASTA | LMBP 9034 |
| pUBQ10:MSCP-mVenus-NLS-NPTII | LMBP 9035 |