Last data update: 24 January 2024 16:39 CET
Plasmid name: p416-SHS-TI (LMBP 11195)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p11195.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Candida albicans SAT1 gene disruption cassette Saccharomyces cerevisiae 2µ plasmid site-specific recombinase Flp cDNA (FLP1); with Candida albicans TUB2 intron |
Promoter: | Candida albicans elongation factor 1-alpha 2 promoter (TEF2) Candida albicans alpha-glucosidase promoter (MAL2) Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) Candida albicans actin promoter (ACT1) |
Ribosome binding site: |
- |
Terminator: | Candida albicans ATP-dependent 6-phosphofructokinase subunit beta terminator (PFK2) Candida albicans actin terminator (ACT1) |
Selection marker: | Ampicillin (amp) Hygromycin (hyg) Saccharomyces cerevisiae URA3; auxotrophic |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4 |
Host range: | Candida albicans; ura3(-) Saccharomyces cerevisiae; ura3(-) Escherichia coli |
Parental clone: | p416-SHS |
Further information: | The plasmid was constructed by cloning the Candida albicans TUB2 intron into the FLP coding sequence of p416-SHS. The plasmid is intended for gene deletion in Candida albicans. The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MF048870.1. |
EMBL Accession number: | MF048870.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 26/03/2019 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | The plasmid was deposited by Dr K. Ganesan(1). It was constructed by Dr S. Bijlani(1). (1) CSIR Institute of Microbial Technology, Chandigarh, India |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
Distributed as: | active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
p416-SHS-TI (LMBP 11195) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.