Last data update: 24 January 2024 16:39 CET
Plasmid name: pACpLpA (LMBP 4236)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p4236.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human adenovirus 5 genome (Ad5); fragment |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Human adenovirus 5 origin (Ad5) |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pACCMVpLpA |
| Further information: | The vector was constructed by deletion of the cytomegalovirus (CMV) early gene promoter/enhancer out of the pACCMVpLpA vector. This plasmid is used in combination with e.g. pJM17 to generate recombinant Ad5 adenovirus. Other name of the plasmid is pLpa-CMV. |
| EMBL Accession number: | X02996, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 01/08/2001 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AflIII, AflIII/EcoNI, AflIII/NotI, BamHI/SspI, BglI, EcoRI, HindII, NcoI, NdeI, NotI, SacI, SpeI and SspI. The restriction site analysis pattern revealed that SspI is not unique, in contrast with the reconstructed nucleotide sequence. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr B. De Geest(1). (1) Department of Transgene Technology and Gene Therapy, KUL, Leuven, Belgium |
| Plasmid reference: | De Geest et al., Hum. Gene Ther. 11 (2000), 101-112 [PMID: 10646643] |
| Related plasmid reference: | Gomez-Foix et al., J. Biol. Chem. 267 (1992), 25129-25134 [PMID: 1334082] Becker et al., Methods Cell Biol. 43 (1994), 161-189 [PMID: 7823861] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pACpLpA (LMBP 4236) is available at BCCM/GeneCorner. This plasmid was deposited by Dr B. De Geest and was published in De Geest et al., 2000. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.