Last data update: 24 January 2024 16:39 CET
Plasmid name: pBlueBacmsGCB1-H105F (LMBP 4643)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p4643.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse guanylate cyclase 1, soluble, beta 3 cDNA (Gucy1b3, beta 1 sGC, sGCB1); mutated sequence |
| Promoter: | Autographa californica nuclear polyhedrosis virus (AcNPV) polyhedrin promoter (PH) Autographa californica nuclear polyhedrosis virus (AcNPV) EcoRI-T large promoter (ETL) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Insect cells; e.g. Sf9 cells |
| Parental clone: | pBlueBacmsGCB1 |
| Further information: | The plasmid was constructed via site-directed mutagenesis of pBlueBacmsGCB1, introducing a H105F mutation and a unique BalI restriction site. Other name of the plasmid is pBlueBac4.5/V5-msGCBeta_H105F. |
| EMBL Accession number: | - |
| Latest sequence update: | 12/12/2002 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII, EcoRV, HaeII and HindIII/SacI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Brouckaert(1) (2). It was constructed by M. Buys(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 TOP10 |
| Host reference: | Instruction Manual (Invitrogen) |
| Related host reference: | Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBlueBacmsGCB1-H105F (LMBP 4643) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Brouckaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.