Last data update: 24 January 2024 16:39 CET
Plasmid name: pCXP22h (LMBP 10204)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p10204.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Histidine tag (His-tag); C-terminal |
Promoter: | Synthetic prokaryotic P22 promoter |
Ribosome binding site: |
- |
Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pTrc99A; pVIK165 P22; pTrcHis-TOPO; pGEM-T |
Further information: | The construction of this plasmid is described in Aerts et al. (2011). The plasmid is meant for constitutive expression in E. coli via the intermediate P22 promoter, in order to optimize enzyme expression for high-throughput screening. Other names of the plasmid are pCXhP22 and pCXP22h empty vector. |
EMBL Accession number: | - |
Latest sequence update: | 06/12/2016 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | The plasmid was deposited by Prof. Dr T. Desmet(1). It was constructed by Dr D. Aerts(1). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium |
Plasmid reference: | Aerts et al., Eng. Life Sci. 11 (2011), 10-19 [DOI: 10.1002/elsc.201000065] |
Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCXP22h (LMBP 10204) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr T. Desmet and was published in Aerts et al., 2011. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.