Last data update: 24 January 2024 16:39 CET
Plasmid name: pD603-HA-LZTR1-R466Q (LMBP 11169)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11169.gb |
| Sequence analysis results Genecorner: |
NGS: g04-gc-dec2018.fasta |
| Cloned DNA: |
Human leucine zipper like transcription regulator 1 cDNA (LZTR1, LZTR-1, BTBD29, GeneID 8216); mutated sequence Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal |
| Promoter: | Simian virus 40 early promoter (SV40 early) Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCMV |
| Further information: | The plasmid was constructed by cloning the synthetic HA-tagged mutant human LZTR1 coding sequence into the pCMV vector. The human LZTR1 coding sequence contains an R466Q mutation. The remaining nucleotide sequence of the human LZTR1 coding sequence was altered for ease of synthesis but contains no codon changes. Other name of the plasmid is pD603 HA-LZTR1 R466Q (1525). |
| EMBL Accession number: | - |
| Latest sequence update: | 24/01/2019 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr Anna Sablina(1)(2). (1) Center for Cancer Biology, VIB, Leuven, Belgium (2) Department of Oncology, University of Leuven, Leuven, Belgium |
| Plasmid reference: | Steklov et al., Science 362 (2018), 1177-1182 [PMID: 30442762] [DOI: 10.1126/science.aap7607] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pD603-HA-LZTR1-R466Q (LMBP 11169) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Anna Sablina and was published in Steklov et al., 2018. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.