Last data update: 24 January 2024 16:39 CET
Plasmid name: pDEST-LZRS-mRIP1-ires-eGFP (LMBP 11335)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse receptor TNFRSF-interacting serine-threonine kinase 1 cDNA (Ripk1, Rip1, Rinp, GeneID 19766) Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP) Epstein-Barr virus nuclear antigen 1 gene (EBNA1, EBNA-1, GeneID 3783709) |
| Promoter: | Moloney mouse leukemia virus long terminal repeat (5' MMLV-LTR) |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Moloney mouse leukemia virus long terminal repeat (3' MMLV-LTR) |
| Selection marker: | Ampicillin (amp) Puromycin (puro) |
| Replicon: | Escherichia coli plasmid pMB1 origin Epstein-Barr virus origin of replication (oriP) |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pDEST-LZRS-ires-eGFP |
| Further information: | The plasmid was constructed by cloning the mouse Ripk1 coding sequence into the pDEST-LZRS-ires-eGFP vector. This retroviral Gateway expression vector carries the EBNA1 gene which is a trans-acting factor that allows episomal maintenance of oriP-bearing plasmids. It is the only virally encoded protein necessary for plasmid maintenance. The nucleotide sequence of the mouse Ripk1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number BC058162.1. |
| EMBL Accession number: | BC058162.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 25/11/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Vandenabeele(1) (2). It was constructed by Dr L. Duprez(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 in E. coli; L2 in mammalian cells |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pDEST-LZRS-mRIP1-ires-eGFP (LMBP 11335) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Vandenabeele . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.