Last data update: 24 January 2024 16:39 CET
Plasmid name: pDMIphoAE6L (LMBP 2960)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2960.gb
(View with Genome Compiler) p2960.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli alkaline phosphatase A gene (phoA); signal sequence Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; light chain (E6L) missing the leader signal sequence Escherichia coli lac repressor gene (lacI) |
| Promoter: | Escherichia coli hybrid tryptophan/lacUV5 promoter (tac) Escherichia coli lac repressor promoter; mutant (lacI(q)) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA) |
| Terminator: | - |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid p15A origin |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | pDMI,1; pBRphoAE6L |
| Further information: | The plasmid was constructed by ligation of three fragments: 1) the XhoI-NruI fragment from pBRphoAE6L, containing on the one hand the Escherichia coli alkaline phosphatase A (phoA) signal sequence fused in phase to cDNA encoding the light chain of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ), E6L, missing the leader signal sequence, and on the other hand the tac promoter; 2) the large NruI-MluI fragment from pDMI,1, containing the neomycin resistance gene, the p15A origin of replication, the lacI(q) repressor promoter and the 5' part of the lacI repressor gene; 3) the MluI-SalI fragment from pDMI,1, containing the remaining part of the lacI repressor gene. The plasmid is used for co-expression with plasmids encoding the heavy chain of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ), E6H. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727563.1. Name mentioned in Flamez et al. (1995) is pDMI,1E6L. Other name of the plasmid is pDMI,1phoAE6L. |
| EMBL Accession number: | LT727563.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 23/02/1994 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BssHII, EcoRV, PstI and StyI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr K. De Sutter(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Flamez et al., J. Biotechnol. 42 (1995), 133-143 [PMID: 7576533] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pDMIphoAE6L (LMBP 2960) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and was published in Flamez et al., 1995. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.