Last data update: 24 January 2024 16:39 CET
Plasmid name: pEBBK3 (LMBP 2567)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | pebbk3.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | BK virus early promoter (BKV early) BK virus late promoter (BKV late) Herpes simplex virus (HSV) thymidine kinase promoter (TK) |
| Ribosome binding site: |
- |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin Epstein-Barr virus origin of replication (oriP) BK virus origin (BKV) |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | p220.2; pBODE |
| Further information: | The origin of the BK virus was isolated from pBode as a HindIII-fragment and inserted into the unique HindIII-site of p220.2. Orientation of late BK-transcription is similar to the orientation of the hyg-gene of p220.2 (and opposite relative to pEBBK3). EBNA1 (Epstein-Bar virus nuclear antigen 1) is a Gly-Ala protein, coded by the triplets GGA, GGG and GCA. This repeat sequence lies in the BKRF1 (EBNA1) reading frame (pos. 418 to 2343). The location of the promoter is not yet known but may be located several kilobases upstream of the start of the BKRF1 reading frame. EBNA1 is a trans-acting factor which allows extrachromosomal maintenance of oriP-bearing plasmids. OriP is a region of EBV DNA permitting linked DNAs to replicate as plasmids in cells containing EBV DNA (EBNA1 gene). The bacterial gene for Hygromycin B resistance is expressed using the promoter and polyadenylation signals of the Herpes simplex thymidine kinase gene. The sequence of the Hyg gene was taken from 'Genbank Release 55' (Gritz and Davies (1983)) and differs in one nucleotide from the sequence on the restriction map given by Sugden (pos. 415 : T in 'Genbank', C by Sugden). Other names of the plasmid are pBode richt B kl5 and p220.2BK2. |
| EMBL Accession number: | - |
| Latest sequence update: | 16/06/1994 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEBBK3 (LMBP 2567) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.