Last data update: 24 January 2024 16:39 CET
Plasmid name: p220.2 (LMBP 1966)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1966.gb
(View with Genome Compiler) p1966.txt p1966.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Epstein-Barr virus nuclear antigen 1 gene (EBNA1, EBNA-1, GeneID 3783709) |
| Promoter: | Herpes simplex virus (HSV) thymidine kinase promoter (TK) |
| Ribosome binding site: |
- |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin Epstein-Barr virus origin of replication (oriP) |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | p201 |
| Further information: | This plasmid is an EBNA1 expression plasmid. EBNA1 is a trans-acting factor which allows episomal maintenance of oriP-bearing plasmids. It is the only virally encoded protein necessary for plasmid maintenance. The protein contains DNA-linking domains which contribute to the activation of transcription and replication, a DNA-binding and dimerization domain, a chromosome binding domain and a gly-ala repeat. The central domain of EBNA1 is entirely composed of a repetitive array of glycine and alanine residues. The majority of this domain can be deleted without affecting the EBNA1 function. The bacterial gene for hygromycin B resistance is expressed under control of the promoter and polyadenylation signals of the Herpes simplex thymidine kinase gene. Addition of hygromycin to the cell line cultivation medium prevents the decrease of p220.2 copies. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727609.1. |
| EMBL Accession number: | LT727609.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 16/06/1992 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AvaI, EcoRI and EcoRV. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr B. Sugden(1). (1) McArdle Laboratory for Cancer Research, Wisconsin, USA |
| Plasmid reference: | Yates et al., Nature 313 (1985), 812-815 [PMID: 2983224] |
| Related plasmid reference: | Van Craenenbroeck et al., Gene 253 (2000), 293-301 [PMID: 10940567] Van Craenenbroeck et al., Eur. J. Biochem. 267 (2000), 5665-5678 [PMID: 10971576] Sugden et al., Mol. Cell. Biol. 5 (1985), 410-413 [PMID: 2983194] McKnight, Nucleic Acids Res. 8 (1980), 5949-5964 [PMID: 6258156] Gritz et al., Gene 25 (1983), 179-188 [PMID: 6319235] Baer et al., Nature 310 (1984), 207-211 [PMID: 6087149] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
p220.2 (LMBP 1966) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr B. Sugden and was published in Yates et al., 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.