Last data update: 24 January 2024 16:39 CET
Plasmid name: pEBSV3 (LMBP 2340)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2340.gb
(View with Genome Compiler) p2340.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Epstein-Barr virus nuclear antigen 1 gene (EBNA1, EBNA-1, GeneID 3783709) |
| Promoter: | Simian virus 40 late promoter (SV40 late) Simian virus 40 early promoter (SV40 early) Herpes simplex virus (HSV) thymidine kinase promoter (TK) |
| Ribosome binding site: |
- |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Hygromycin (hyg) Ampicillin (amp) |
| Replicon: | Epstein-Barr virus origin of replication (oriP) Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; e.g. HelaH21, MG63, CV-1, HepG2, 293 |
| Parental clone: | p220.2; pSV55i1 |
| Further information: | The plasmid was constructed as follows: the BglII-BclI fragment, containing the SV40 late promoter, of pSV55i1 was inserted anticlockwise into the unique BamHI site of p220.2. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727504.1. Other name of the plasmid is p220.2SV2. |
| EMBL Accession number: | LT727504.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 29/04/1991 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindIII, XbaI and XmaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEBSV3 (LMBP 2340) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vanhoenacker and Prof. Dr G. Haegeman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.