Last data update: 24 January 2024 16:39 CET
Plasmid name: pEBSV3cat (LMBP 3039)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3039.gb
(View with Genome Compiler) p3039.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Chloramphenicol acetyltransferase gene (cat) Epstein-Barr virus nuclear antigen 1 gene (EBNA1, EBNA-1, GeneID 3783709) |
| Promoter: | Simian virus 40 late promoter (SV40 late) Simian virus 40 early promoter (SV40 early) Herpes simplex virus (HSV) thymidine kinase promoter (TK) |
| Ribosome binding site: |
- |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Hygromycin (hyg) Ampicillin (amp) |
| Replicon: | Epstein-Barr virus origin of replication (oriP) Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; e.g. HelaH21, MG63, CV-1, HepG2, 293 |
| Parental clone: | pEBSV3; pBLCAT5 |
| Further information: | The plasmid was constructed as follows: the XhoII fragment (795 bp), containing the chloramphenicol acetyltransferase gene, of pBLCAT5 was cloned into the BamHI-opened pEBSV3 vector. The orientation is such that the cat gene is under control of the SV40 late promoter. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727423.1. |
| EMBL Accession number: | LT727423.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/08/1994 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, MluI/XhoI and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEBSV3cat (LMBP 3039) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vanhoenacker and Prof. Dr G. Haegeman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.