Last data update: 24 January 2024 16:39 CET
Plasmid name: pEBSV3d (LMBP 2733)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2733.gb
(View with Genome Compiler) p2733.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Epstein-Barr virus nuclear antigen 1 gene (EBNA1, EBNA-1, GeneID 3783709) |
| Promoter: | Simian virus 40 late promoter (SV40 late) Simian virus 40 early promoter (SV40 early) Herpes simplex virus (HSV) thymidine kinase promoter (TK) |
| Ribosome binding site: |
- |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Hygromycin (hyg) Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Epstein-Barr virus origin of replication (oriP) |
| Host range: | Escherichia coli Mammalian cells; e.g. HelaH21, MG63, CV-1, HepG2, 293 |
| Parental clone: | p220.2; pMKSV40/8-4 |
| Further information: | The plasmid was constructed as follows: the Sau3AI fragment, containing the origin region of SV40 (Gluzman mutant), of p8-4 was inserted anticlockwise into the unique BamHI site of p220.2. This plasmid contains a non-functional origin of SV40 and is used for studies concerning the stability of the SV40 origin in EBV containing vectors. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727541.1. Other name of the plasmid is pEBGluz2. |
| EMBL Accession number: | LT727541.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 23/09/1994 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AvaI, EcoRV/NgoMIV and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Gluzman et al., Proc. Natl. Acad. Sci. U.S.A. 77 (1980), 3898-3902 [PMID: 6254000] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEBSV3d (LMBP 2733) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vanhoenacker and Prof. Dr G. Haegeman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.