Last data update: 24 January 2024 16:39 CET
Plasmid name: pECFP-p65 (LMBP 11490)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11490.gb |
| Sequence analysis results Genecorner: |
NGS: a04-gc-dec2019.fasta |
| Cloned DNA: |
Human RELA proto-oncogene, NF-kB subunit (RELA, NFKB3, p65, GeneID 5970) Aequorea victoria green fluorescent protein DNA (GFP); enhanced cyan fluorescent variant (ECFP), N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) Escherichia coli β-lactamase promoter (amp) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Phage f1 origin Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pECFP-C1 |
| Further information: | The plasmid was constructed by cloning the human RELA coding sequence into the pECFP-C1 vector, downstream of the ECFP marker. The start ATG codon of the human RELA coding sequence was removed. The plasmid is useful for FRET analysis. |
| EMBL Accession number: | - |
| Latest sequence update: | 13/06/2019 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by S. Dewaele(1) (2) and Prof. Dr C. Libert(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Dendoncker et al., Proc. Natl. Acad. Sci. U.S.A. 116 (2019), 12942-12951 [PMID: 31182584] [DOI: 10.1073/pnas.1821565116] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pECFP-p65 (LMBP 11490) is available at BCCM/GeneCorner. This plasmid was deposited by S. Dewaele and Prof. Dr C. Libert and was published in Dendoncker et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.