Last data update: 24 January 2024 16:39 CET
Plasmid name: pEF-BOS-HA-IRF3Gal4 (LMBP 5275)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5275.gb |
| Sequence analysis results Genecorner: |
NGS: e02-gc-may2021.fasta |
| Cloned DNA: |
Human interferon regulatory factor 3 (IRF3, GeneID 3661) Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) |
| Promoter: | Human elongation factor 1α promoter (EF1α) Escherichia coli lac operon promoter Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Human granulocyte macrophage colony stimulating factor polyadenylation signal (GM-CSF polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Bacteriophage M13 origin Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pEF-HA-IRF3 |
| Further information: | The plasmid was constructed by cloning the S. cerevisiae GALbd coding sequence into the pEF-HA-IRF3 vector. This plasmid is useful to test human IRF3 for its ability to activate a Gal4-responsive reporter. |
| EMBL Accession number: | - |
| Latest sequence update: | 08/06/2006 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr E. Aksoy(1). (1) William Harvey Research Institute, Queen Mary University of London, London, United Kingdom |
| Plasmid reference: | Yoneyama et al., EMBO J. 17 (1998), 1087-1095 [PMID: 9463386] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEF-BOS-HA-IRF3Gal4 (LMBP 5275) is available at BCCM/GeneCorner. This plasmid was deposited by Dr E. Aksoy and was published in Yoneyama et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.