Last data update: 24 January 2024 16:39 CET
Plasmid name: pEF6crmA (LMBP 5107)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5107.gb
(View with Genome Compiler) p5107.txt p5107.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Cowpox virus cytokine response modifier A cDNA (crmA) Myc epitope; C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Human elongation factor 1α promoter (EF1α) Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Synthetic prokaryotic EM7 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Blasticidin (bsd) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli; preferably recombination-deficient and endonuclease A deficient strains Mammalian cells; SV40 permissive cells |
| Parental clone: | pEF6/Myc-HisA; pCAGGScrmA |
| Further information: | The plasmid was constructed by inserting the EcoRI fragment of pCAGGScrmA, containing the crmA cDNA, in the EcoRI opened pEF6/Myc-HisA vector. As a result, the C-terminal Myc epitope and histidine tag are not expressed. The plasmid is designed for high-level expression of crmA under control of the hEF1α promoter in mammalian cells. The plasmid carries the ampicillin resistance gene for selection in E. coli and the blasticidin resistance gene for selection in both E. coli and mammalian cells. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727088.1. The nucleotide sequence of the crmA cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M14217.1. Other name of the plasmid is pEF6/Myc-HisACrmA. |
| EMBL Accession number: | M14217.1, view at EMBL, GenBank, DDBJ LT727088.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 07/11/2006 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, NdeI and NruI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Vandenabeele(1) (2). It was constructed by E. Van Damme(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEF6crmA (LMBP 5107) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Vandenabeele . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.