Last data update: 24 January 2024 16:39 CET
Plasmid name: pEGFP-IkB (LMBP 8293)
| New search | Print data sheet |
| Price category: | Cat. 2 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8293.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Pig/human nuclear factor κB (NF-κB) inhibitor alpha hybrid cDNA (IκBα, NFKBIA, MAD-3); human N-terminal fragment Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP), N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli β-lactamase promoter (amp) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pEGFP-C1 |
| Further information: | The plasmid was constructed by cloning a human/pig hybrid NFKBIA CDS into the pEGFP-C1 vector. The human/pig hybrid NFKBIA CDS was obtained by fusing the N-terminal 241 nt of human NFKBIA to the C-terminal 704 nt of pig NFKBIA. The nucleotide sequence of the human NFKBIA cDNA fragment corresponds with the EMBL Nucleotide Sequence Database accession number BC004983.1. The nucleotide sequence of the pig NFKBIA cDNA fragment corresponds with the Genbank accession number NM_001005150.1. |
| EMBL Accession number: | BC004983.1, view at EMBL, GenBank, DDBJ NM_001005150.1, view at GenBank |
| Latest sequence update: | 13/03/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AgeI, HaeII, HincII and HindIII/HpaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr J.A. Schmid(1). (1) Center for Physiology and Pharmacology, Medical University Vienna, Vienna, Austria |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEGFP-IkB (LMBP 8293) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J.A. Schmid. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.