Last data update: 24 January 2024 16:39 CET
Plasmid name: pEGFP-N1-TRAP1 (LMBP 6239)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p6239.gb
(View with Genome Compiler) p6239.txt p6239.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human TNF receptor-associated protein 1 cDNA (TRAP1); mature sequence Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP), C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli β-lactamase promoter (amp) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pEGFP-N1 |
| Further information: | The plasmid was constructed by cloning the human TRAP1 cDNA as an EcoRI-BamHI fragment into the pEGFP-N1 vector. The human TRAP1 cDNA is lacking the first 58 codons, thereby eliminating its mitochondrial localisation signal. The EGFP cds is fused to the C-terminus of TRAP1. The depositor has tested this construct in transient transfections: TRAP1 goes to the cytoplasm as judged by fluorescence microscopy and cellular fractionation. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726828.1. The N-terminal region of the human TRAP1 cds, downstream from the pMB1 ori, and the C-terminal region of the human TRAP1 cds, upstream of the SV40 polyA signal, were sequenced by LMBP. The nucleotide sequence of the human TRAP1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF154108.1. The nucleotide sequence of the pEGFP-N1 vector corresponds with the EMBL Nucleotide Sequence Database accession number U55762.1. The nucleotide sequence of the EGFP DNA corresponds with the EMBL Nucleotide Sequence Database accession number U55761.1. |
| EMBL Accession number: | U55762.1, view at EMBL, GenBank, DDBJ U55761.1, view at EMBL, GenBank, DDBJ AF154108.1, view at EMBL, GenBank, DDBJ LT726828.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 04/09/2012 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/EcoRI, BglI, BstXI/EcoRI and NotI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr S. Felts(1). (1) Department of Immunology, Mayo Clinic College of Medicine, Rochester, Minnesota, USA |
| Plasmid reference: | - |
| Related plasmid reference: | Felts et al., J. Biol. Chem. 275 (2000), 3305-3312 [PMID: 10652318] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEGFP-N1-TRAP1 (LMBP 6239) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Felts. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.