Last data update: 24 January 2024 16:39 CET
Plasmid name: pEGFP-fliC-1-52 (LMBP 9780)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9780.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Salmonella enterica phase 1 flagellin cDNA (fliC); N-terminal fragment Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP), N-terminal |
| Promoter: | Escherichia coli β-lactamase promoter (amp) Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pEGFP-C1 |
| Further information: | The plasmid was constructed by cloning an N-terminal fragment of the S. enterica fliC coding sequence, containing codons 1 up to and including 52, into the pEGFP-C1 vector. Protein expression of this vector in HEK293T cells was tested at GeneCorner. The nucleotide sequence of the S. enterica fliC cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AY353420.1. The nucleotide sequence of the EGFP DNA corresponds with the EMBL Nucleotide Sequence Database accession number U55761.1. Other name of the plasmid is pEGFP-1-52 |
| EMBL Accession number: | AY353420.1, view at EMBL, GenBank, DDBJ U55761.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/01/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr M. Matusiak (1)(2) and Prof. Dr M. Lamkanfi (1)(2). (1) Department of Internal Medicine, UGent, Gent, Belgium (2) Inflammation Research Center, VIB, Zwijnaarde, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEGFP-fliC-1-52 (LMBP 9780) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Matusiak and Prof. Dr M. Lamkanfi . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.