Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR1A-crmA (LMBP 6824)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p6824.gb
(View with Genome Compiler) p6824.txt p6824.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Cowpox virus cytokine response modifier A cDNA (crmA) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pCAGGScrmA; pENTR1A |
| Further information: | This Gateway entry vector was created by isolating the cowpox virus crmA cDNA from pCAGGScrmA as an EcoRI fragment and cloning it into the EcoRI opened pENTR1A vector. The nucleotide sequence of the cowpox virus crmA cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M14217.1. Other name of the plasmid is pEntry1A CrmA WT. |
| EMBL Accession number: | M14217.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 25/02/2011 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, NsiI/SalI, PvuI and PvuII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR1A-crmA (LMBP 6824) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.