Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR207-PRS-hRIP3-K50A (LMBP 8655)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8655.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human receptor interacting serine/threonine kinase 3 cDNA (RIPK3, RIP3, GeneID 11035); mutated sequence Human rhinovirus 3C protease cleavage site (PreScission site, PRS); N-terminal |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Gentamicin (Gm) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pENTR207-PRS-hRIP3 |
| Further information: | This Gateway entry vector was constructed by introducing a K50A mutation in the human RIPK3 coding sequence of pENTR207-PRS-hRIP3. The human RIPK3 coding sequence is preceded by a PreScission protease cleavage site. The nucleotide sequence of the wild type human RIPK3 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF156884.1. Other name of the plasmid is pDONR207-PRS-hRIP3K50A(FL)+stop. |
| EMBL Accession number: | AF156884.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 24/08/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr P. Vandenabeele(1)(2). It was constructed by I. Bruggeman(1)(2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + gentamicin (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR207-PRS-hRIP3-K50A (LMBP 8655) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr P. Vandenabeele. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.