Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR207-PRS-mRIP1-K45R(KD+ID) (LMBP 8644)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p8644.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Mouse receptor TNFRSF-interacting serine-threonine kinase 1 cDNA (Ripk1, Rip1, Rinp, GeneID 19766); mutated fragment Human rhinovirus 3C protease cleavage site (PreScission site, PRS); N-terminal |
Promoter: | - |
Ribosome binding site: |
- |
Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
Selection marker: | Gentamicin (Gm) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pDONR207 |
Further information: | This Gateway entry vector was introducing a K45R mutatin in the kinase domain (KD) and intermediate domain (ID) of the mouse Ripk1 coding sequence and cloning it into the pDONR207 vector via Gateway BP recombination. The plasmid contains the kinase domain (KD) and intermediate domain (ID) of the mouse Ripk1 coding sequence, preceded by a PreScission protease cleavage site. The nucleotide sequence of the wild type mouse Ripk1 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number BC058162.1. Other name of the plasmid is pDONR207-PRS-mRIP1K45R (KD+ID)+stop. |
EMBL Accession number: | BC058162.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 24/08/2016 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | The plasmid was deposited by Prof. Dr P. Vandenabeele(1)(2). It was constructed by I. Bruggeman(1)(2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + gentamicin (10 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR207-PRS-mRIP1-K45R(KD+ID) (LMBP 8644) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr P. Vandenabeele. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.