Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR221-hPKCbeta1-no_stop (LMBP 10015)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10015.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human protein kinase C beta cDNA (PRKCB, PKCB, PRKCB1, PRKCB2, PKCbeta, GeneID 5579); transcript variant 1 |
| Promoter: | Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pDONR221 |
| Further information: | This Gateway entry vector was constructed by PCR amplifying the human PRKCB transcript variant 1 coding sequence, without its termination codon, with primers attaching an attB1 and attB2 site, and cloning it into the pDONR221 vector via Gateway BP recombination. The nucleotide sequence of the human PRKCB transcript variant 1 coding sequence corresponds with the Genbank accession number NM_212535.2. Other name of the plasmid is pENT221_hPKCbeta1 -Stop. |
| EMBL Accession number: | NM_212535.2, view at GenBank |
| Latest sequence update: | 13/10/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr W. Declercq(1)(2). It was constructed by K. Leurs(1)(2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Corinne Urwyler |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR221-hPKCbeta1-no_stop (LMBP 10015) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr W. Declercq and was published in Corinne Urwyler, . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.