Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR3C-GFP-mPARK2-mut (LMBP 8689)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8689.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse parkin RBR E3 ubiquitin protein ligase cDNA (Prkn, Park2, Parkin, GeneID 50873); mutated sequence Aequorea victoria green fluorescent protein DNA (GFP); N-terminal |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pENTR3C |
| Further information: | This Gateway entry vector was constructed by cloning the mouse Park2 mutated sequence N-terminally fused to the GFP coding sequence into the pENTR3C vector. The mouse Park2 coding sequence contains 4 mutations: the G nucleotide on position 96 of the wild type CDS is replaced by a T, resulting in a K32N amino acid change; there is an additional GCA codon between nucleotide positions 411 and 412, introducing an additional alanine amino acid; the C nucleotide on position 1140 of the wild type CDS is replaced by a T, but does not result in an amino acid change; the G nucleotide on position 1192 of the wild type CDS is replaced by a C, resulting in an E398Q amino acid change. The nucleotide sequence of the wild type mouse Park2 coding sequence corresponds with the Genbank accession number NM_016694.4. Other name of the plasmid is pENTR3C-GFP-mParkin+stop (with mutations). |
| EMBL Accession number: | NM_016694.4, view at GenBank |
| Latest sequence update: | 24/08/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr P. Vandenabeele(1)(2). It was constructed by I. Bruggeman(1)(2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR3C-GFP-mPARK2-mut (LMBP 8689) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr P. Vandenabeele. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.