Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR3C-hCARMA2-H171N (LMBP 9625)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p9625.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human caspase recruitment domain family member 14 cDNA (CARD14, BIMP2, CARMA2, PSORS2, GeneID 79092); mutated sequence |
Promoter: | - |
Ribosome binding site: |
- |
Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
Selection marker: | Neomycin (neo; kanamycin (kan)) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pCDNA3.1-FLAG-hCARD14-H171N; pENTR3C |
Further information: | The plasmid was constructed by PCR amplifying the human CARD14 mutated sequence from pCDNA3.1-FLAG-hCARD14-H171N and cloning it into the pENTR3C vector. The human CARD14 coding sequence contains an H171N mutation. The nucleotide sequence of the wild type human CARD14 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF322642.1. |
EMBL Accession number: | AF322642.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 28/02/2018 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. R. Beyaert(1) (2). (1) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR3C-hCARMA2-H171N (LMBP 9625) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.