Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR3C-hSLC12A6 (LMBP 8643)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p8643.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human solute carrier family 12 member 6 cDNA (SLC12A6, ACCPN, KCC3) |
Promoter: | - |
Ribosome binding site: |
- |
Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
Selection marker: | Neomycin (neo; kanamycin (kan)) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pENTR3C |
Further information: | This Gateway entry plasmid was constructed by cloning the human SLC12A6, transcript variant 3 coding sequence, without a termination codon, into the pENTR3C vector. The nucleotide sequence of the human SLC12A6 coding sequence corresponds with the Genbank accession number AL359474.7, except for nucleotides 1415 up to and including 1417 which are missing, a G to A nucleotide change in position 2334 and nucleotides 2760 up to and including 2788 which are missing, resulting in a frameshift and truncation of the encoded protein. Other name of the plasmid is pENTR3C-hSLC12A6V3. |
EMBL Accession number: | AL359474.7, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 24/08/2016 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | The plasmid was deposited by Prof. Dr P. Vandenabeele(1)(2). It was constructed by I. Bruggeman(1)(2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR3C-hSLC12A6 (LMBP 8643) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr P. Vandenabeele. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.